isolating dna experiment matriculation

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SB015 Pre-Lab Module

EXPERIMENT 6: BASIC TECHNIQUE IN ISOLATING DNA

Course Learning Outcome: Solve problems related to cells, biomolecules, inheritance, genetics and biological development. (C4, PLO 4, CTPS3, MQF LOD 6)

Learning Outcomes: At the end of this lesson, students should be able to: i. State the basic concept of DNA and its importance. ii. State the functions of the materials used to prepare DNA isolation. iii. Explain the correct technique of handling apparatus and materials.

Student Learning Time: Face-to-face Non face-to-face 1 hour 1 hour

Direction : Read over the lab manual and then answer the following questions.

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Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence f or use in justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. Imagine you are a scientist who is assigned to collect samples and isolate the DNA in a laboratory.

Click the URL and conduct the DNA extraction process. Read the instructions on what to do in each slide. Use the “next” and “back” button to get from slide to slide. learn.genetics.utah/content/labs/extraction/

Introduction

State THREE organelles in plant cells which contain DNA.
What is the function of DNA?

What are chromosomes made of?

Extraction of DNA in plant cells nucleus differ from animal cells. Briefly discuss.

Why is it important for scientists to be able to remove DNA from organism? List TWO reasons.

It is important that you understand the steps in the extraction procedure and why each step was necessary. Each step in the procedure aided in isolating the DNA from other cellular materials. Match the following procedure with the function.

A. Filter fruits mixture through muslin cloth ____ to precipitate DNA from solution B. Add mash fruits with salt-detergent solution

____ separate components of the cell

C. Initial smashing and grinding of fruits ____ break open the cell D. Addition of alcohol to filtered extract membranes

____ break up proteins and dissolve cell